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(solution) Determine the sequence of a linear, unbranched decapeptide for


Determine the sequence of a linear, unbranched decapeptide for which the following experiments have been done:

0. Amino acid analysis gives one each of the following 10 amino acids:

Trp, Glx, Met, Arg, Asx, Cys, Phe, Pro, Thr, His, 1 NH4+

 1. Edman?s reagent gave a PTH derivative indicative of Gln

2. Cyanogen bromide (CNBr) cleavage gives two pentapeptides.

3. V8 protease (cleaves after Asp/Glu) gives a heptapeptide that absorbs at 280nm and a tripeptide. When the tripeptide is treated with ninhydrin, a weakly colored yellow compound is obtained. The tripeptide has a charge of 0 at pH 5.0.

4. Trypsin cleavage gives a tetrapeptide that absorbs at 280 and a hexapeptide

5. Chymotrypsin cleavage gives a dipeptide that absorbs at 260 nm, a single amino acid that absorbs strongly at 280 nm, and a heptapeptide.

6. The C-terminal residue is identified as Cys using carboxypeptidase Y followed by phenylisothiocyanate treatment

 => Give the sequence in three-letter code separated by dashes. Example Ala-Ala-Val-Ile. Must contain 10 amino acids from the list above from N-terminal to C-terminal.

Make sure there is no typo, or you will get zero point. Only a perfect match will give you points.

 


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