Determine the sequence of a linear, unbranched decapeptide for which the following experiments have been done:
0. Amino acid analysis gives one each of the following 10 amino acids:
Trp, Glx, Met, Arg, Asx, Cys, Phe, Pro, Thr, His, 1 NH4+
1. Edman?s reagent gave a PTH derivative indicative of Gln
2. Cyanogen bromide (CNBr) cleavage gives two pentapeptides.
3. V8 protease (cleaves after Asp/Glu) gives a heptapeptide that absorbs at 280nm and a tripeptide. When the tripeptide is treated with ninhydrin, a weakly colored yellow compound is obtained. The tripeptide has a charge of 0 at pH 5.0.
4. Trypsin cleavage gives a tetrapeptide that absorbs at 280 and a hexapeptide
5. Chymotrypsin cleavage gives a dipeptide that absorbs at 260 nm, a single amino acid that absorbs strongly at 280 nm, and a heptapeptide.
6. The C-terminal residue is identified as Cys using carboxypeptidase Y followed by phenylisothiocyanate treatment
=> Give the sequence in three-letter code separated by dashes. Example Ala-Ala-Val-Ile. Must contain 10 amino acids from the list above from N-terminal to C-terminal.
Make sure there is no typo, or you will get zero point. Only a perfect match will give you points.
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