(solution) Receptor Pharmacology worksheet. Please help me solve this

(solution) Receptor Pharmacology worksheet. Please help me solve this

Receptor Pharmacology worksheet. Please help me solve this pharmacodynamics / experimental pharmacology problem.

A membrane prep was assayed for NET (NE transporter) binding using increasing concentrations of 3H-Nisoxetine in triplicate.
Background binding for each reaction was identified in duplicate using 10 mM desipramine Sample tube#
1 2 3 4 5 DPM
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25 total bckgrd 837
1329
924
189
168
1530
1398
1737
234
249
5061
4668
3660
867
753
7470
7527
7824
771
873
9552
8763
8547
1290
1242 specific mg protein
per Rxn dpm/mg fmol/mg 0.364 0.306 0.377 0.325 0.436 Please answer the following questions:
What is the average total dpm for each reaction?
What is the average "background" dpm for each reaction?
What is the average specific dpm for each reaction?
What is the specific dpm/mg for each reaction?
calculate the fmol/mg of receptor for each reaction, using the following information:
Specific Activity of the 3H-Nisoxetine= 87.2 Ci/mmol
There are 2.2 x 10^6 dpm/mCi [Hint: make the units cancel]
Show your work for converting dpm/mg to fmol/mg – you can get partial credit even if the answer is wrong
Graph the resulting fmol/mg (you can use excel or another graphing program). What is your ballpark estimate of the Bmax?